The effective epi ethanol extract of T. chebula dried fruit selected based on the preliminary
studies of phytochemicals, antioxidant (ABTS and DPPH), in-vitro antibacterial and
antifungal activity. The chemical compounds present in epi ethanol extract were identified,
along with their structure, using GCMS. The nature of compounds and carbon environment
were determined through NMR spectroscopy. The toxicity of phytochemicals in epi ethanol
extract tested by seed germination studies, the observations revealed the absence of any toxic
compounds in the ethanol extract instead it increases the seed germination rate (bio
stimulant), Seed vigour index and germination percentage at 0.1mg/mL. The metabolic
protein and protease activity were higher in the extract-treated seeds than in the control seeds.
The host tomato (L. esculentum) was used to investigate the allelopathic effects of epi ethanol
extract through pot culture in-vivo study. None of the results showed the presence of
inhibitory compounds in ethanol extract on the tomato plants instead it stimulated the growth.
The important plant pathogen R. solanacearum was selected for disease severity to test the
effect of ethanol extract on induced disease plant. The incidence of bacterial wilt in tomatoes
reduced notably in inoculated and treated plants proving that ethanol extracts (0.1mg/mL)
was safe and can be used to control the BW in tomatoes. The epi ethanol extract found to be
safe for consumers and environment based on the docking studies. Mainly five chemical
compounds in ethanol extract bind strongly to enzymes Dihydrolipoamide dehydrogenase
and citrate synthase of R. solanacearum and leading to death.
– Dr. Padmalatha Ajith